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SARS-Like Coronavirus WIV1-CoV Does Not Replicate in Egyptian Fruit Bats (Rousettus aegyptiacus)

Identifieur interne : 000C42 ( Main/Exploration ); précédent : 000C41; suivant : 000C43

SARS-Like Coronavirus WIV1-CoV Does Not Replicate in Egyptian Fruit Bats (Rousettus aegyptiacus)

Auteurs : Neeltje Van Doremalen ; Alexandra Sch Fer ; Vineet D. Menachery ; Michael Letko ; Trenton Bushmaker ; Robert J. Fischer ; Dania M. Figueroa ; Patrick W. Hanley ; Greg Saturday ; Ralph S. Baric ; Vincent J. Munster

Source :

RBID : PMC:6316779

Descripteurs français

English descriptors

Abstract

Severe acute respiratory syndrome (SARS)-like WIV1-coronavirus (CoV) was first isolated from Rhinolophus sinicus bats and can use the human angiotensin converting enzyme 2 (ACE2) receptor. In the current study, we investigate the ability of WIV1-CoV to infect Rousettus aegyptiacus bats. No clinical signs were observed throughout the experiment. Furthermore, only four oropharyngeal swabs and two respiratory tissues, isolated on day 3 post inoculation, were found positive for viral RNA. Two out of twelve bats showed a modest increase in coronavirus specific antibodies post challenge. In conclusion, WIV1-CoV was unable to cause a robust infection in Rousettus aegyptiacus bats.


Url:
DOI: 10.3390/v10120727
PubMed: 30572566
PubMed Central: 6316779


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<title xml:lang="en" level="a" type="main">SARS-Like Coronavirus WIV1-CoV Does Not Replicate in Egyptian Fruit Bats (
<italic>Rousettus aegyptiacus</italic>
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<author>
<name sortKey="Van Doremalen, Neeltje" sort="Van Doremalen, Neeltje" uniqKey="Van Doremalen N" first="Neeltje" last="Van Doremalen">Neeltje Van Doremalen</name>
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<name sortKey="Sch Fer, Alexandra" sort="Sch Fer, Alexandra" uniqKey="Sch Fer A" first="Alexandra" last="Sch Fer">Alexandra Sch Fer</name>
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<nlm:aff id="af2-viruses-10-00727">Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA;
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<name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D." last="Menachery">Vineet D. Menachery</name>
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<nlm:aff id="af2-viruses-10-00727">Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA;
<email>aschaefe@email.unc.edu</email>
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<email>vimenach@UTMB.EDU</email>
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<name sortKey="Letko, Michael" sort="Letko, Michael" uniqKey="Letko M" first="Michael" last="Letko">Michael Letko</name>
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<name sortKey="Bushmaker, Trenton" sort="Bushmaker, Trenton" uniqKey="Bushmaker T" first="Trenton" last="Bushmaker">Trenton Bushmaker</name>
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<name sortKey="Fischer, Robert J" sort="Fischer, Robert J" uniqKey="Fischer R" first="Robert J." last="Fischer">Robert J. Fischer</name>
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<nlm:aff id="af1-viruses-10-00727">Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;
<email>neeltje.vandoremalen@nih.gov</email>
(N.v.D.);
<email>michael.letko@nih.gov</email>
(M.L.);
<email>bushmakertj@niaid.nih.gov</email>
(T.B.);
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<name sortKey="Figueroa, Dania M" sort="Figueroa, Dania M" uniqKey="Figueroa D" first="Dania M." last="Figueroa">Dania M. Figueroa</name>
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<nlm:aff id="af1-viruses-10-00727">Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;
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(N.v.D.);
<email>michael.letko@nih.gov</email>
(M.L.);
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(T.B.);
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<email>dania.figueroa@nih.gov</email>
(D.M.F.)</nlm:aff>
</affiliation>
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<author>
<name sortKey="Hanley, Patrick W" sort="Hanley, Patrick W" uniqKey="Hanley P" first="Patrick W." last="Hanley">Patrick W. Hanley</name>
<affiliation>
<nlm:aff id="af3-viruses-10-00727">Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;
<email>patrick.hanley@nih.gov</email>
(P.W.H.);
<email>greg.saturday@nih.gov</email>
(G.S.)</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Saturday, Greg" sort="Saturday, Greg" uniqKey="Saturday G" first="Greg" last="Saturday">Greg Saturday</name>
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<nlm:aff id="af3-viruses-10-00727">Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;
<email>patrick.hanley@nih.gov</email>
(P.W.H.);
<email>greg.saturday@nih.gov</email>
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</affiliation>
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<name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name>
<affiliation>
<nlm:aff id="af2-viruses-10-00727">Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA;
<email>aschaefe@email.unc.edu</email>
(A.S.);
<email>vimenach@UTMB.EDU</email>
(V.D.M.);
<email>rbaric@email.unc.edu</email>
(R.S.B.)</nlm:aff>
</affiliation>
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<name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J." last="Munster">Vincent J. Munster</name>
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(N.v.D.);
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(M.L.);
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(T.B.);
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<term>Animals</term>
<term>Antibodies, Viral (blood)</term>
<term>Chiroptera (virology)</term>
<term>Coronavirus (genetics)</term>
<term>Coronavirus (physiology)</term>
<term>Coronavirus Infections (immunology)</term>
<term>Coronavirus Infections (virology)</term>
<term>Disease Models, Animal</term>
<term>Male</term>
<term>Oropharynx (virology)</term>
<term>RNA, Viral (genetics)</term>
<term>SARS Virus (genetics)</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>ARN viral (génétique)</term>
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<term>Coronavirus (génétique)</term>
<term>Coronavirus (physiologie)</term>
<term>Infections à coronavirus (immunologie)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Modèles animaux de maladie humaine</term>
<term>Mâle</term>
<term>Partie orale du pharynx (virologie)</term>
<term>Réplication virale</term>
<term>Virus du SRAS (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en">
<term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Coronavirus</term>
<term>RNA, Viral</term>
<term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>ARN viral</term>
<term>Coronavirus</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr">
<term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en">
<term>Coronavirus Infections</term>
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<term>Coronavirus</term>
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<term>Coronavirus</term>
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<term>Anticorps antiviraux</term>
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<term>Chiroptera</term>
<term>Infections à coronavirus</term>
<term>Partie orale du pharynx</term>
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<term>Virus Replication</term>
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<term>Animaux</term>
<term>Modèles animaux de maladie humaine</term>
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<front>
<div type="abstract" xml:lang="en">
<p>Severe acute respiratory syndrome (SARS)-like WIV1-coronavirus (CoV) was first isolated from
<italic>Rhinolophus sinicus</italic>
bats and can use the human angiotensin converting enzyme 2 (ACE2) receptor. In the current study, we investigate the ability of WIV1-CoV to infect
<italic>Rousettus aegyptiacus</italic>
bats. No clinical signs were observed throughout the experiment. Furthermore, only four oropharyngeal swabs and two respiratory tissues, isolated on day 3 post inoculation, were found positive for viral RNA. Two out of twelve bats showed a modest increase in coronavirus specific antibodies post challenge. In conclusion, WIV1-CoV was unable to cause a robust infection in
<italic>Rousettus aegyptiacus</italic>
bats.</p>
</div>
</front>
<back>
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<author>
<name sortKey="De Wit, E" uniqKey="De Wit E">E. De Wit</name>
</author>
<author>
<name sortKey="Van Doremalen, N" uniqKey="Van Doremalen N">N. van Doremalen</name>
</author>
<author>
<name sortKey="Falzarano, D" uniqKey="Falzarano D">D. Falzarano</name>
</author>
<author>
<name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name>
</author>
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<author>
<name sortKey="Lau, S K" uniqKey="Lau S">S.K. Lau</name>
</author>
<author>
<name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name>
</author>
<author>
<name sortKey="Li, K S" uniqKey="Li K">K.S. Li</name>
</author>
<author>
<name sortKey="Huang, Y" uniqKey="Huang Y">Y. Huang</name>
</author>
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